Refereed Journal Articles

Book Chapters

Yeo, A.T., Rawal, S., Delcuze, B., Christofides, A., Atayde, A., Strauss, L., Balaj, L., Rogers, V.A., Uhlmann, E.J., Varma, H., Carter, B.S., Boussiotis, V.A. and Charest, A., Single-cell RNA sequencing reveals evolution of immune landscape during glioblastoma progression. Nat Immunol, 2022. 23(6): p. 971-984. PMC9174057.  10.1038/s41590-022-01215-0.

Mateescu, B., Jones, J.C., Alexander, R.P., Alsop, E., An, J.Y., Asghari, M., Boomgarden, A., Bouchareychas, L., Cayota, A., Chang, H.C., Charest, A., Chiu, D.T., Coffey, R.J., Das, S., De Hoff, P., deMello, A., D'Souza-Schorey, C., Elashoff, D., Eliato, K.R., Franklin, J.L., Galas, D.J., Gerstein, M.B., Ghiran, I.H., Go, D.B., Gould, S., Grogan, T.R., Higginbotham, J.N., Hladik, F., Huang, T.J., Huo, X., Hutchins, E., Jeppesen, D.K., Jovanovic-Talisman, T., Kim, B.Y.S., Kim, S., Kim, K.M., Kim, Y., Kitchen, R.R., Knouse, V., LaPlante, E.L., Lebrilla, C.B., Lee, L.J., Lennon, K.M., Li, G., Li, F., Li, T., Liu, T., Liu, Z., Maddox, A.L., McCarthy, K., Meechoovet, B., Maniya, N., Meng, Y., Milosavljevic, A., Min, B.H., Morey, A., Ng, M., Nolan, J., De Oliveira Junior, G.P., Paulaitis, M.E., Phu, T.A., Raffai, R.L., Reategui, E., Roth, M.E., Routenberg, D.A., Rozowsky, J., Rufo, J., Senapati, S., Shachar, S., Sharma, H., Sood, A.K., Stavrakis, S., Sturchler, A., Tewari, M., Tosar, J.P., Tucker-Schwartz, A.K., Turchinovich, A., Valkov, N., Van Keuren-Jensen, K., Vickers, K.C., Vojtech, L., Vreeland, W.N., Wang, C., Wang, K., Wang, Z., Welsh, J.A., Witwer, K.W., Wong, D.T.W., Xia, J., Xie, Y.H., Yang, K., Zaborowski, M.P., Zhang, C., Zhang, Q., Zivkovic, A.M. and Laurent, L.C., Phase 2 of extracellular RNA communication consortium charts next-generation approaches for extracellular RNA research. iScience, 2022. 25(8): p. 104653. PMC9358052.  10.1016/j.isci.2022.104653.

Christofides, A., Strauss, L., Yeo, A., Cao, C., Charest, A. and Boussiotis, V.A., The complex role of tumor-infiltrating macrophages. Nat Immunol, 2022. 23(8): p. 1148-1156.  10.1038/s41590-022-01267-2.

Charest, A., Optimizing an effective combination of the new microtubule-targeting agent lisavanbulin with standard-of-care therapy for glioblastoma in patient-derived xenograft preclinical models. Neuro Oncol, 2022. 24(3): p. 396-397. PMC8917392.  10.1093/neuonc/noab278.

Yeo, A.T., Jun, H.J., Appleman, V.A., Zhang, P., Varma, H., Sarkaria, J.N. and Charest, A., EGFRvIII tumorigenicity requires PDGFRA co-signaling and reveals therapeutic vulnerabilities in glioblastoma. Oncogene, 2021. 40(15): p. 2682-2696. PMC9159289.  10.1038/s41388-021-01721-9.

Zeng, A., Wei, Z., Rabinovsky, R., Jun, H.J., El Fatimy, R., Deforzh, E., Arora, R., Yao, Y., Yao, S., Yan, W., Uhlmann, E.J., Charest, A., You, Y. and Krichevsky, A.M., Glioblastoma-Derived Extracellular Vesicles Facilitate Transformation of Astrocytes via Reprogramming Oncogenic Metabolism. iScience, 2020. 23(8): p. 101420. PMC7424213.  10.1016/j.isci.2020.101420.

Vidal, B., Bryke, C., Hsu, N., Alterman, R., Yeo, A.T., Charest, A. and Varma, H., A high-grade glioma with SOS1 amplification. Clin Neuropathol, 2020. 39(3): p. 126-134.  10.5414/NP301182.

Ko, J., Wang, Y., Carlson, J.C.T., Marquard, A., Gungabeesoon, J., Charest, A., Weitz, D., Pittet, M.J. and Weissleder, R., Single Extracellular Vesicle Protein Analysis Using Immuno-Droplet Digital Polymerase Chain Reaction Amplification. Adv Biosyst, 2020. 4(12): p. e1900307. PMC8491538.  10.1002/adbi.201900307.

Charest, A., Experimental and Biological Insights from Proteomic Analyses of Extracellular Vesicle Cargos in Normalcy and Disease. Adv Biosyst, 2020. 4(12): p. e2000069. PMC8091982.  10.1002/adbi.202000069.

Jones, P.S., Yekula, A., Lansbury, E., Small, J.L., Ayinon, C., Mordecai, S., Hochberg, F.H., Tigges, J., Delcuze, B., Charest, A., Ghiran, I., Balaj, L. and Carter, B.S., Characterization of plasma-derived protoporphyrin-IX-positive extracellular vesicles following 5-ALA use in patients with malignant glioma. EBioMedicine, 2019. 48: p. 23-35. PMC6838454.  10.1016/j.ebiom.2019.09.025.

Gyuris, A., Navarrete-Perea, J., Jo, A., Cristea, S., Zhou, S., Fraser, K., Wei, Z., Krichevsky, A.M., Weissleder, R., Lee, H., Gygi, S.P. and Charest, A., Physical and Molecular Landscapes of Mouse Glioma Extracellular Vesicles Define Heterogeneity. Cell Rep, 2019. 27(13): p. 3972-3987 e6. PMC6604862.  10.1016/j.celrep.2019.05.089.

Das, S., Extracellular, R.N.A.C.C., Ansel, K.M., Bitzer, M., Breakefield, X.O., Charest, A., Galas, D.J., Gerstein, M.B., Gupta, M., Milosavljevic, A., McManus, M.T., Patel, T., Raffai, R.L., Rozowsky, J., Roth, M.E., Saugstad, J.A., Van Keuren-Jensen, K., Weaver, A.M. and Laurent, L.C., The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research. Cell, 2019. 177(2): p. 231-242. PMC6601620.  10.1016/j.cell.2019.03.023.

Bardhan, K., Aksoylar, H.I., Le Bourgeois, T., Strauss, L., Weaver, J.D., Delcuze, B., Charest, A., Patsoukis, N. and Boussiotis, V.A., Phosphorylation of PD-1-Y248 is a marker of PD-1-mediated inhibitory function in human T cells. Sci Rep, 2019. 9(1): p. 17252. PMC6872651.  10.1038/s41598-019-53463-0.

Jun, H.J., Appleman, V., Wu, HJ., Rose, C., Pineda, JJ., Yeo, A., Delcuze, B., Lee, C., Gyuris, A., Zhu, H., Woolfenden, S., Bronisz, A., Nakano, I., Chiocca, E.A., Bronson, R.T., Ligon, K., Sarkaria, J.N., Gygi, S., Michor, F., Mitchison, T. and Charest, A. α sensitivity to Vinblastine. 2018. Nat Commun. 2018 Aug 6;9(1):3116. PMCID: PMC6078993

Zhou, S., Appleman, V.A., Rose, C.M., Jun, H.J., Yang, J., Zhou, Y., Bronson, R.T., Gygi, S.P. and Charest, A., Chronic platelet-derived growth factor receptor signaling exerts control over initiation of protein translation in glioma. Life Sci Alliance, 2018. 1(3): p. e201800029. PMC6238596.  10.26508/lsa.201800029.

Boussiotis, V.A. and Charest, A. Immunotherapies for Malignant Glioma. Invited Review, Oncogene. 2018. Mar; 37(9):1121-1141. PMCID: PMC5828703

Yeo, A. and Charest, A. Immune Checkpoint Blockade Biology in Mouse Models of Glioblastoma. Invited Review, J Cell Biochem. 2017. 118(9): 2516-2527. PMCID: PMC5783695

Ricklefs, F., Mineo, M., Nakano, I., Charest, A., Weissleder, R., Breakefield, X. O., Chiocca, E. A., Godlewski, J. and Bronisz, A. High grade glioma extracellular vesicles retain subtype specificity and transmit intratumoral pro-oncogenic signals. Can Res., 2016, 76(10):2876-81. PMCID: PMC4873326.

Seib, F.P., Coburn, J., Konrad, I., Klebanov, N., Jones, G.T., Blackwood, B., Charest, A., Kaplan, D,L,, Chiu, B. Focal therapy of neuroblastoma using silk films to deliver kinase and chemotherapeutic agents in vivo. Acta Biomater. 2015 Jul;20:32-8. PMCID: PMC4428956

Zou, H.Y., Lia, Q., Engstrom, L.D., Lu, M.W., Appleman, V., Wong, K.A., McTigue, M., Deng, Y.L., Liu, W., Brooun, A., Timofeevski, S., McDonnell, S., Jiang, P., Falk, M.D., Lappin, P.B., Affolter, T., Nicholes, T., Hu, W., Lam, J., Johnson, T.W., Smeal, T., Charest, A*., Fantin, V.R*. PF-06463922 is a potent and selective next generation ROS1/ALK inhibitor capable of blocking crizotinib resistant ROS1 mutations. 2015. P.N.A.S. 112(11):3493-8. * Equal Contribution. PMCID: PMC4371934

Ali, Z., de Jesus Perez, V., Yuan, K., Orcholski, M., Pan, S., Qi, W., Chopra, G., Adams, C., Kojima, Y., Leeper, N., Qu, X., Zaleta-Rivera, K., Kato, K., Yamada, Y., Oguri, M., Kuchinsky, A., Hazen, S.L., Jukema, W.,Ganesh, S., Nabel, E., Channon, K., Leon, M., Charest, A., Quertermous, T., Ashley, E. Oxido-reductive regulation of human vascular remodeling by the orphan receptor tyrosine kinase ROS1. 2014. J. Clin. Invest. 124(12):5159-74. PMCID: PMC4348976.

Jun H.J., Roy J., Smith, T.B., Wood, L.B., Lane, K., Woolfenden, S., Punko, D., Bronson, R.T., Haigis, K.M., Breton, S., Charest, A. ROS1 Signaling Regulates Epithelial Differentiation in the Epididymis. 2014. Endocrinology. 155(9):3661-73. PMCID: PMC4138574.

Chiu, B., Coburn, J., Pilichowska, M., Holcroft, C., Seib, F.P., Charest, A., Kaplan, D.L. Surgery Combined with Controlled-Release Doxorubicin Silk Films as a Treatment Strategy in an Orthotopic Neuroblastoma Mouse Model. 2014. Brit Jour Cancer, 111(4):708-15. PMCID: PMC4134491.

Jun HJ, Bronson RT, Charest A. Inhibition of EGFR Induces a c-MET Driven Stem Cell Population in Glioblastoma. Stem Cells. 2014. 32(2):338-48. PMCID: PMC4442493.

Jun HJ, Johnson H, Bronson RT, de Feraudy S, White F, Charest A. The Oncogenic Lung Cancer Fusion Kinase CD74-ROS Activates a Novel Invasiveness Pathway Through E-Syt1 Phosphorylation. Cancer Res. 2012. 72(15):3764-74. PMCID: PMC3753671

Shao, H., Chung, J., Balaj, L., Charest, A., Bigner, D.D., Carter, B.S., Hochberg, F.H., Breakefield, X.O., Weissleder, R., Lee, H. Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy. Nature Medicine. 2012. 18(12):1835-40. PMCID: PMC3518564

Jun, H.J., Acquaviva, J., Chi, D., Lessard, J., Zhu, H., Woolfenden, S., Bronson, R.T., Pfannl, R., White, F., Housman, D.E., Iyer, L., Whittaker, C.A., Boskovitz, A., Raval, A., Charest, A. Acquired MET Expression Confers Resistance to EGFR Inhibition In a Mouse Model of Glioblastoma Multiforme. Oncogene. 2012. 31(25):3039-50. PMCID: PMC3774279

Acquaviva, J., Jun, H.J., Lessard, J., Ruiz, R., Zhu, H., Donovan, M., Woolfenden, S., Boskovitz, A., Raval, A., Bronson, R.T., Pfannl, R., Whittaker, C.A., Housman, D.E., Charest, A. Chronic Activation of Wild Type Epidermal Growth Factor Receptor and Loss of Cdkn2a Cause Mouse Glioblastoma Formation. Can Res. 2011. Dec 1;71(23):7198-206. PMCID: PMC3228869

Zhu, H., Woolfenden. S., Bronson, R.T., Jaffer, Z.M., Barluenga, S., Winssinger, N., Rubenstein, A.E., Chen, R., Charest, A. The novel Hsp90 inhibitor NXD30001 induces tumor regression in a genetically engineered mouse model of glioblastoma multiforme. Mol Cancer Ther. 2010. (9):2618-26. PMCID: PMC2937276

Gutmann, D.H., Stiles, C.D., Lowe, S.W., Bollag, G.E., Furnari, F.B., Charest, A. Report from the Fifth National Cancer Institute Mouse Models of Human Cancers Consortium Nervous System Tumors Workshop. Neuro Oncology. 2011. 13(7):692-9. PMCID: PMC3129277

Hambardzumyan, D., Parada, L.F., Holland, E.C., Charest, A. Genetic modeling of gliomas in mice: new tools to tackle old problems. Glia. 2011. 59(8):1155-68. PMCID: PMC3619979

Woolfenden, S., Zhu, H., Charest, A. A Cre/LoxP Conditional Luciferase Reporter Transgenic Mouse for Bioluminescence Monitoring of Tumorigenesis. Genesis. 2009. 47(10):659-666. Pubmed.

Agrawal, A., Min, D-H., Zhu, H., Birjiniuk, A., von Maltzahn, G., Harris, T.J., Xing, D., Woolfenden, S., Sharp, P.A., Charest, A., Bhatia, S. Functional Delivery of siRNA in Mice using Dendriworms. ACS Nano. 2009. 3(9):2495-504. PMCID: PMC2828182

Jun, H.J., Woolfenden, S., Coven, S., Lane, K., Bronson, R., Housman, D., Charest, A. Epigenetic regulation of c-ros receptor tyrosine kinase expression in malignant gliomas. Cancer Research. 2009. 69(6):2180-4. Pubmed.

Zhu, H., Acquaviva, J., Ramachandran, H., Boskovitz, A., Woolfenden, S., Pfannl, R, Bronson, R., Chen, J., Weissleder, R., Housman, D., Charest, A. Oncogenic EGFR Signaling Cooperates with Loss of Tumor Suppressor Gene Functions in Gliomagenesis. Proc Natl Acad Sci U S A. 2009. 106(8):2712-16. PMCID: PMC2650331

Acquaviva, J., Wong, R., Charest, A. The multifaceted roles of the receptor tyrosine kinase ROS in development and cancer. BBA Rev Can. 2009. 1795, pp. 37-52. Pubmed.

Daniel, K.G., Kim, G.Y., Vassiliou, C.C., Galindo, M., Guimaraes, A.R., Charest, A., Langer, R., Cima, M.J. Implantable Diagnostic Device for Cancer Sensing. 2009. Biosens Bioelectron. 24(11):3252-7. PMCID: PMC2738970

Mills, J., Hippo, Y., Robert, F., Chen, M-H., Malina, A., Lin, C.-J., Trojahn, U.,  Wendel, H.-G., Charest, A., Bronson, R.T., Kogan, S.C., Nadon, R., Housman, D., Lowe, S.W., Pelletier, J. 2008  mTORC1 promotes survival through translational control of Mcl-1. Proc Natl Acad Sci U S A. Aug 5;105(31): 10853-8. PMCID: PMC2504845

Sirois, J., Côté, JF., Charest, A., Uetani, N., Bourdeau, A., Duncan, SA., Daniels, E., and Tremblay, ML. Essential function of PTP-PEST during mouse embryonic vascularization, mesenchyme formation, neurogenesis and early liver development. Mech Dev. 2006 Dec;123(12):869-80. Pubmed.

Kissil, JL., Charest, A., New Tools for Dissecting RON Receptor Tyrosine Kinase Oncogenic Signaling. Cancer Biol Ther. 2006 Sep 11;5(9). Pubmed.

Charest, A., Wilker, E., McLaughlin, M., Lane, K., Gowda, R., Coven, S., McMahon, K., Kovach, S., Feng, Y., Yaffe, M., Jacks, T, Housman, D. 2006. ROS Fusion Tyrosine Kinase Activates a SHP-2/PI3K/mTOR Signaling Axis to Form Glioblastoma in Mice. Cancer Research. 66:7473-7481. Pubmed.

McCarty JH, Lacy-Hulbert A, Charest A, Bronson RT, Crowley D, Housman D, Savill J, Roes J, Hynes RO. 2005. Selective ablation of alphav integrins in the central nervous system leads to cerebral hemorrhage, seizures, axonal degeneration and premature death.Development.132(1):165-76. Pubmed.

Charest A, Lane K, McMahon K, Park J, Preisinger E, Conroy H, Housman D. 2003. Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21). Genes Chromosomes Cancer.37(1):58-71. Pubmed.

Charest A, Kheifets V, Park J, Lane K, McMahon K, Nutt CL, Housman D. 2003. Oncogenic targeting of an activated tyrosine kinase to the Golgi apparatus in a glioblastoma. Proc Natl Acad Sci U S A. 100(3):916-21. Pubmed.

Jordan B, Charest A, Dowd JF, Blumenstiel JP, Yeh Rf RF, Osman A, Housman DE, Landers JE. 2002. Genome complexity reduction for SNP genotyping analysis. Proc Natl Acad Sci U S A. 99(5):2942-7. Pubmed.

Charest A, Lane K, McMahon K, Housman DE. 2001. Association of a novel PDZ domain-containing peripheral Golgi protein with the Q-SNARE (Q-soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor) protein syntaxin 6. J Biol Chem. Aug 3;276(31):29456-65. Pubmed.

Chattopadhyay S, Kaul R, Charest A, Housman D, Chen J. 2000. SMAR1, a novel, alternatively spliced gene product, binds the Scaffold/Matrix-associated region at the T cell receptor beta locus. Genomics. Aug 15;68(1):93-6. Pubmed.

Storring, J. M., Charest, A., Cheng, P., and Albert, P. R. 1999. TATA-driven Transcriptional Initiation and Regulation of the Rat Serotonin 5-HT1A Receptor Gene. J Neurochem. 1999 Jun;72(6):2238-47. Pubmed.

Angers-Loustau A, Cote JF, Charest A, Dowbenko D, Spencer S, Lasky LA, Tremblay ML 1999.  Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts. J Cell Biol;144(5):1019-1031. Pubmed.

Cote JF, Charest A, Wagner J, Tremblay ML 1998. Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model. Biochemistry;37(38):13128-37. Pubmed.

Heesen M, Berman MA, Charest A, Housman D, Gerard C, Dorf ME. 1998. Cloning and chromosomal mapping of an orphan chemokine receptor: mouse RDC1. Immunogenetics;47(5):364-70 . Pubmed.

Charest, A., Wagner, J., Kwan, M., and Tremblay, M. L. 1997. Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2. Oncogene 14: 1643-1651. Pubmed.

Nguyen L., Holgado-Madruga M., Maroun C., Fixman E. D., Kamikura D., Fournier T., Charest A., Tremblay M. L., Wong A. J., Park M. 1997. Association of the multisubstrate docking protein Gab1 with the hepatocyte growth factor receptor requires a functional Grb2 binding site involving tyrosine 1356. J Biol Chem 272(33): 20811-20819. Pubmed.

Charest, A., Wagner, J.,  Jacob, S., McGlade, C. J. ,and Tremblay, M. L. 1996. Phosphotyrosine Independent Binding of SHC to the NPLH Sequence of MPTP-PEST. Evidence for Extended PTB/PI Domain Recognition Specificity. J. Biol. Chem. 271 (14): 8424-8429. Pubmed.

Albert, P.R., Lembo, P., Storring, J., Charest, A., and Saucier, C. 1996. The 5-HT1A receptor: Signalling, Desensitization and Gene Transcription. Neuropsychopharmacology 14: 19-25. Pubmed.

Charest, A., Wagner, J., Muise, E. S., Heng, H. H. Q., and Tremblay, M. L. 1995. Structure of the Murine MPTP-PEST Gene: Genomic Organization and Chromosomal Mapping. Genomics  28 (3): 501-507. Pubmed.

Charest, A., Wagner, J., Shen, S.-H., and Tremblay, M. L. 1995. Murine Protein Tyrosine Phosphatase-PEST: a stable cytosolic protein tyrosine phosphatase. Biochem. J. 308 (pt2): 425-32. Pubmed.

Charest, A., Wainer, B. H., and Albert, P. R. 1993.  Cloning and Differentiation-Induced Expression of a Murine Serotonin1A Receptor in a Septal Cell Line.  J. Neuroscience 13: 5164-5171. Pubmed.

Nanotechnology-based therapy for malignant tumors of the central nervous system. Abraham Boskovitz, Abdullah Kandil and Al Charest. In Tumors of the Central Nervous System. Ed. M. A. Hayat.

EGFR and Tumor–Suppressor Function in Brain Cancer Development. Julie Lessard, M.Sc., Ph.D. and Al Charest, M.Sc., Ph.D. In ANIMAL MODELS OF BRAIN TUMORS. Neuromethods Series, Ed. Wolfgang Walz.

THE RECEPTOR TYROSINE KINASE ROS1. Alain Charest. In The Receptor Tyrosine Kinases Handbook. Ed. Yarden and Wheeler.

Growth Factor Signaling Pathways and Targeted Therapy. Kenneth D. Swanson, Alain Charest, Ian F. Pollack and Eric. T. Wong. Handbook of Brain Tumor Chemotherapy, Molecular Therapeutics, and Immunotherapy. 2018 Elsevier Inc.